[Proteintech] multiproTM Fixed Cell Immune Profiling Antibody Cocktail | 코람바이오텍(주)/코람바이오젠(주)

단일 세포 전사체 분석 기법(Single Cell RNA-sequencing; scRNA-seq)기술과 접목하여 세포 표면 뿐만 아니라 세포 내 단백질 발현까지 분석이 가능한 최초의 상용화된 antibody cocktail 제품을 소개합니다.

multipro^TM Fixed Cell Immune Profiling Antibody Cocktail

Cell surface and intracellular protein를 모두 타깃으로 하는 항체 칵테일입니다.
58개의 Oligonucleotide conjugated monoclonal and polyclonal antibodies로 구성된 동결 건조 칵테일입니다. 자세한 target list는 아래로 스크롤을 내리시면 확인하실 수 있습니다.
(Cellular targets 53개, Isotype control 5개)
본 항체 칵테일은 10x Genomics의 Chromium Single Cell Gene Expression Flex 제품과 호환되어 사용할 수 있으며, Single cell 수준에서 multiple Protein 및 mRNA 데이터를 제공합니다.
Sample Type: Human Fixed cells

Readout:

Next Generation Sequencing – Illumina or Element
Run format: R1 x 48 cycles l Index1 x 10 cycles l Index2 x 10 cycles l R2 x 50 cycles

Kit Format:

4 single-use vials, each tube contains enough lyophilized cocktail to stain 1 x 10⁶ cells.

10x Genomics Required Products:

Product	Size	Cat No.
Next GEM Single Cell Fixed RNA Sample Preparation Kit	16 rxns	PN-1000414
Next GEM Chip Q Single Cell Kit	48 rxns	PN-1000418
Next GEM Chip Q Single Cell Kit	16 rxns	PN-1000422
Fixed RNA Kit, Human Transcriptome	4 rxns x 4 BC	PN-1000475
Fixed RNA Kit, Human Transcriptome	4 rxns x 16 BC	PN-1000476
Fixed RNA Feature Barcode Multiplexing Kit	64 rxns	PN-1000628
Dual Index Kit TS Set A	96 rxns	PN-1000251
Dual Index Kit TN Set A	96 rxns	PN-1000250

multipro^TM Human Fixed Cell Immune Profiling Antibody Cocktail Targets

Secreted	Clone	Expressed by*	Surface	Clone	Expressed by*
IFNG	1E10G7	T cells, NK cells, APCs, ILCs	ANXA2	1C1E12	Basophils, Macrophages, T cells, DCs
IL-1B	2A1B4	Neutrophils, APCs	CD11a	TS2/4	Leukocytes
IL-17A	1F3E3	T cells, APCs, fibroblasts	CD11b	ICRF44	Granulocytes, Monocytes/Macrophages
IL-6	4G3E6	Th17 cells, ILCs, NK cells	CD11c	3.9	DCs, B cells
IL-8	Polyclonal	APCs, Epithelial cells, T cells	CD13	WM15	Granulocytes, Monocytes
S100A4	2G11B4	Leukocytes	CD16	3G8	NK cells, Monocytes, Neutrophils
TGFBI	3E11D11	Leukocytes	CD18	TS1/18	Leukocytes
TNFa	7B8A11	APCs, T cells, NK cells	CD19	HIB19	B cells
Nuclear	Clone	Expressed by*	CD2	TS1/8	NK cells, T cells
AHNAK	Polyclonal	DCs, T cells, basophils	CD21	BU32	B cells
BACH1	2D12A4	Neutrophils, APCs, NK cells	CD27	1C1G3	Treg, T cells, B cells
IRF4	Polyclonal	T cells, APCs	CD28	CD28.2	T cells, NK cells
MAFB	Polyclonal	Monocytes/Macrophages	CD35	E11	B cells, T cells, Monocytes, Granulocytes
NFKB2	6A10E9	Ubiquitous	CD3E	OKT3	T cells
POU2AF1	3C5A7	B cells, Airway epithelial cells	CD4	RPA-T4	pDCs, Monocytes, mDCs, T cells
SPI1	2H3D3	Granulocytes, Monocytes, B cells, NK cells	CD40	G28.5	APCs
T-bet	Polyclonal	NK cells, T cells, B cells	CD43	2A11D6	Leukocytes
Cytosolic	Clone	Expressed by*	CD44	F10-44-2	Ubiquitous
AIF1	Polyclonal	APCs, T cells	CD45	HI30	Lymphocytes
LASP1	1G4B6	Leukocytes	CD45RA	F8-11-13	B and T cells
SRC	5E10C4	T cells, B cells	CD5	UCHT2	T cells
SYK	4C4A12	Monocytes, B cells, NK cells	CD54	15.2	Monocytes, NK cells, DCs
VIM	3H9D1	Mesenchymal cells	CD63	TS63	Basophils, Monocytes, NK cells, T cells
ZAP70	1A11G9	NK cells, T cells, B cells	CD64	10.1	Monocytes, DCs
Controls	Clone		CD7	4H9	NK cell, T cell
Rabbit IgG	Polyclonal		CD71	3C11F11	Monocytes, B cells
Mouse IgG2b	11B8C4		CD81	5A6	Leukocytes
Mouse IgG3	1H4A5		CD83	HB15e	APC B cells
Mouse IgG2a	11A1B2		CD86	BU63	Monocytes, B cells
Mouse IgG1	1F8D3		CD8A	UCHT4	T cells
			HLA-DRA	L243	APCs