Traditional Polyclonal Secondary Antibodies의 단점을 해결할 수 있는
Multi-rAb Recombinant Secondary Antibodies
Multi-rAb Recombinant Secondary Antibodies는 동일한 IgG 상에서 서로 다른 상보적인 epitope을 인식하도록 정교하게 설계된 individual recombinant clone의 혼합물(multiclonal)입니다. 엄격한 검증 과정을 통해 선별된 clone들만 사용하여 항원에 대한 높은 특이성과 민감성을 보장합니다.
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Polyclonal의 high sensitivity와 monoclonal의 superior specificity의 장점이 결합된 2차 항체
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Target species외 minimal cross-reactivity
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Animal Origin Free
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Unlimited supply, high lot-to-lot consistency, reproducibility results
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Validated in WB, ELISA, IHC, ICC and Flow Cytometry
- Species Reactivity: Rabbit, Mouse
- Sizes: 200 μL, 1mL
- Conjugates: HRP, CoraLite® Plus 488, CoraLite® Plus 555, CoraLite® Plus 594, CoraLite® Plus 647, CoraLite® Plus 750
1. How are Multi-rAb Recombinant Secondaries produced?
2. High lot-to-lot consistency
Western blot analysis of various lysates using anti-EIF3E antibody (67095-1-Ig). Three separate batches of Multi-rAb HRP-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM001) were used for detection.
3. Minimal cross-reactivity
Cross reactivity test using Direct ELISA. Rabbit total IgG, Mouse total IgG, Rat total IgG, Pig total IgG, Human total IgG, Bovine total IgG were coated at 100 ng/well. 0.125 μg/mL Multi-rAb HRP-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR001) was used for detection.
4. Optimized performance in every application
Western blot analysis of various lysates using anti-EIF3E antibody (67095-1-Ig). Multi-rAb CoraLite Plus 750-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM006) was used for detection.
Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, green) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, red). Multi-rAb CoraLite® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002, 1:500) and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004, 1:500) were used for detection.
Immunofluorescence of MCF-7 cells: MCF-7 cells were fixed with 4% PFA and stained with Rabbit anti-ZO1 polyclonal antibody (21773-1-AP, 1:2000, orange) and mouse anti-Alpha Tubulin monoclonal antibody (66031-1-Ig, 1:1000, green). Multi-rAb CoraLite® Plus 555-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR003, 1:500) and Multi-rAb CoraLite® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002, 1:500) were used for detection.
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