[ORIGENE] Over-Expression Cell Lysate Controls

Western Blot, ELISA 실험을 위한 검증된 positive controls

Over-Expression Cell lysates are whole-cell lysates of HEK293T cells over-expressing a target protein where the expression of the target protein is confirmed by western blot.

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Over-Expression Lysates 특징

Feature Benefit
A comprehensive library of 20K over-expression lysates covering 80% of the human proteome; includes negative controls One-stop-shop for all positive controls
Proteins expressed in human cells, preserving native protein structure Suitable for antibody screening with a higher probability of detecting antibodies reacting with native epitopes
C-terminal tagged with Myc-DDK Ideal for protein purification
Available in two formats: Frozen and lyophilized Flexible options based on research needs, easy shipping
Protein suspended in non-SDS RIPA buffer Suitable for functional assays, immunoprecipitation, protein-protein interaction studies. Also serve as positive control for antibody screening, western blot, ELISA

Over-Expression Performance Data

Application Data #1: Antibody Validation

Five commercial antibodies against human P53 were evaluated in Western blot experiments with P53 over-expression cell lysate. P53 protein level in cell lysate was pre-determined using a purified GST-Myc-DDK standard. Lysate was serially diluted before SDS-PAGE and immunoblotting. Antibody quality and star rating is based on P53 protein detection level.

Application Data #2: Protein Activity Assay – GATA4 (LY419558)

https://www.korambiotech.com/wp-content/uploads/2022/03/Application-Data-2.jpgDNA-binding activity of GATA4 was measured in OriGene’s over- expression lysate LY419558 and a control lysate. Three microliters of each lysate was tested with a transcription factor binding assay utilizing GATA4-specific DNA sequences. The high level of activity observed in the over-expression lysate compared to the control lysate demonstrates that the expressed GATA4 is biologically active in the lystate.

 ABI1  CELF1  FGFR1  OSBPL9
 ABLIM2  CERKL  FGFR4  P2RX2
 ACRV1  CHID1  FGL1  PAX2
 AMZ2  CHRM2  FHL1  PCBP2
 ANAPC11  CLDN14  GABRA1  PDE9A
 APOL3  CLDND1  HMGA1  PPP2R2B
 APTX  CREM  HNRNPF  PQBP1
 ARSA  CRISP2  ICAM2  PRKAR1B
 ATF3  CSF1  IL32  RAD17
 ATXN3  CSF2RA  IRF5  RBFOX1
 AURKA  CSTF1  MAPT  RNASE9
 B3GALT5  CTSB  MBNL1  RNH1
 BANP  DCAF4  MBP  SCP2
 BCAT1  DCX  MME  SERPINA1
 BDNF  DGKA  MYADM  SIGLEC6
 BIN1  DGLUCY  NAT1  TCF7L2
 CA1  DHRS9  NCALD  TGIF1
 CABYR  DISC1  NDRG2  TPM1
 CACNB2  DMD  NEK6  TSPAN4
 CAMK2B  DTNA  NR1I3  TTC23
 CAMKK2  EEF1D  NRG1  UBE2D3
 CASP7  ETV1  NUDT1  YWHAZ
 CCM2  EYA1  OPA1  ZBTB20
 CD1E  FAM111A  OPRM1  ZFYVE27
 CD59  FCGR2B  OPTN  ZNF200

HEK293T

Control Cell Lysate

Cat No. Description
LY500001 HEK293 cell lysate – no transient overexpression (negative control)
LY500002 Transient Overexpression Lysate of Myc-DDK-tGFP tags
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