Western Blot, ELISA 실험을 위한 검증된 positive controls
Over-Expression Cell lysates are whole-cell lysates of HEK293T cells over-expressing a target protein where the expression of the target protein is confirmed by western blot.
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Over-Expression Lysates 특징
|A comprehensive library of 20K over-expression lysates covering 80% of the human proteome; includes negative controls||One-stop-shop for all positive controls|
|Proteins expressed in human cells, preserving native protein structure||Suitable for antibody screening with a higher probability of detecting antibodies reacting with native epitopes|
|C-terminal tagged with Myc-DDK||Ideal for protein purification|
|Available in two formats: Frozen and lyophilized||Flexible options based on research needs, easy shipping|
|Protein suspended in non-SDS RIPA buffer||Suitable for functional assays, immunoprecipitation, protein-protein interaction studies. Also serve as positive control for antibody screening, western blot, ELISA|
Over-Expression Performance Data
Application Data #1: Antibody Validation
Five commercial antibodies against human P53 were evaluated in Western blot experiments with P53 over-expression cell lysate. P53 protein level in cell lysate was pre-determined using a purified GST-Myc-DDK standard. Lysate was serially diluted before SDS-PAGE and immunoblotting. Antibody quality and star rating is based on P53 protein detection level.
Application Data #2: Protein Activity Assay – GATA4 (LY419558)
DNA-binding activity of GATA4 was measured in OriGene’s over- expression lysate LY419558 and a control lysate. Three microliters of each lysate was tested with a transcription factor binding assay utilizing GATA4-specific DNA sequences. The high level of activity observed in the over-expression lysate compared to the control lysate demonstrates that the expressed GATA4 is biologically active in the lystate.
Control Cell Lysate
|LY500001||HEK293 cell lysate – no transient overexpression (negative control)|
|LY500002||Transient Overexpression Lysate of Myc-DDK-tGFP tags|