[ORIGENE] Over-Expression Cell Lysate Controls


Western Blot, ELISA 실험을 위한 검증된 positive controls

Over-Expression Cell lysates are whole-cell lysates of HEK293T cells over-expressing a target protein where the expression of the target protein is confirmed by western blot.

Over-Expression Lysates는 어떻게 만들어지나요?

Over-Expression Lysates 특징

Feature Benefit
A comprehensive library of 20K over-expression lysates covering 80% of the human proteome; includes negative controls One-stop-shop for all positive controls
Proteins expressed in human cells, preserving native protein structure Suitable for antibody screening with a higher probability of detecting antibodies reacting with native epitopes
C-terminal tagged with Myc-DDK Ideal for protein purification
Available in two formats: Frozen and lyophilized Flexible options based on research needs, easy shipping
Protein suspended in non-SDS RIPA buffer Suitable for functional assays, immunoprecipitation, protein-protein interaction studies. Also serve as positive control for antibody screening, western blot, ELISA

Over-Expression Performance Data

Application Data #1: Antibody Validation


Five commercial antibodies against human P53 were evaluated in Western blot experiments with P53 over-expression cell lysate. P53 protein level in cell lysate was pre-determined using a purified GST-Myc-DDK standard. Lysate was serially diluted before SDS-PAGE and immunoblotting. Antibody quality and star rating is based on P53 protein detection level.

Application Data #2: Protein Activity Assay – GATA4 (LY419558)

https://www.korambiotech.com/wp-content/uploads/2022/03/Application-Data-2.jpgDNA-binding activity of GATA4 was measured in OriGene’s over- expression lysate LY419558 and a control lysate. Three microliters of each lysate was tested with a transcription factor binding assay utilizing GATA4-specific DNA sequences. The high level of activity observed in the over-expression lysate compared to the control lysate demonstrates that the expressed GATA4 is biologically active in the lystate.

 ATF3  CSF1  IL32  RAD17


Control Cell Lysate

Cat No. Description
LY500001 HEK293 cell lysate – no transient overexpression (negative control)
LY500002 Transient Overexpression Lysate of Myc-DDK-tGFP tags
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