RNA Extraction
1. Monarch® Total RNA Miniprep Kit (NEB #T2010)
Developed for total RNA extraction from several sample types, this kit can also be used for viral RNA extraction.
2. Monarch RNA Cleanup Kits (NEB #T2040 and #T2030)
Traditionally used for cleanup applications, these kits have now been validated for extraction, expanding its utility to support viral detection.
Virus Detection
1. Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB# E3019)
The Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit is a real-time RT-PCR assay for the qualitative detection of SARS-CoV-2 nucleic acid.
2. Luna RT-qPCR Kits
Luna Universal One-Step RT-qPCR kits offer exceptional sensitivity, reproducibility and RT-qPCR performance. Available for probe- or dye-based detection, Luna kits utilize a novel, thermostable WarmStart RT, which increases reaction specificity. Reaction mixes also contain dUTP, which supports carryover prevention.
- Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019)
- Luna Universal Probe One-Step RT-qPCR Kit (NEB #E3006)
- Luna Probe One-Step RT-qPCR Kit (No ROX) (NEB #E3007)
- Luna Cell Ready Lysis Module (NEB #E3032)
3. SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (NEB# E2019)
The SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit utilizes loop-mediated isothermal amplification for use in the analysis of SARS-CoV-2, the novel coronavirus that causes COVID-19.
4. WarmStart Colorimetric LAMP 2X Master Mix (DNA & RNA) (NEB #M1800)
WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution
Sequencing & Epidemiology
1. NEBNext ARTIC products for Illumina® and Oxford Nanopore Technologies® sequencing
NEBNext ARTIC kits are based on the original work of the ARTIC Network. The kits include balanced ARTIC primer pools for improved uniformity of genome coverage, from 10-10,000 SARS-CoV-2 genome copies, and reagents for RT-PCR and library prep that are optimized for the SARS-CoV-2 ARTIC workflow.
- NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (for ~150 bp libraries) (NEB #E7658)
- NEBNext ARTIC SARS-CoV-2 Library Prep Kit (Illumina) (for ~400 bp libraries) (NEB #E7650)
- NEBNext ARTIC SARS-CoV-2 Companion Kit (Oxford Nanopore Technologies) (NEB #E7660)
Viral Biology
1. Furin (NEB # P8077)
Furin is a ubiquitous subtilisin-like proprotein convertase with a minimal cleavage site of Arg-X-X-Arg˅. The enzyme prefers the site Arg-X-Lys/Arg-Arg˅.
2.Trypsin-ultra™, Mass Spectrometry Grade (NEB # P8101S)
Trypsin-ultra, Mass Spectrometry Grade is a serine endopeptidase, which selectively cleaves peptide bonds C-terminal to lysine and arginine residues.
3. Endoproteinase LysC (NEB # P8109S)
Endoproteinase LysC is a serine endoproteinase, which cleaves peptide bonds at the carboxyl side of lysine.
Vaccine Development
Reagents for mRNA synthesis
NEB’s portfolio of research-grade and GMP-grade* reagents enables bench-scale to commercial-scale mRNA manufacturing, enabling a seamless transition to large-scale therapeutic mRNA manufacturing.
