[NEB] WarmStart® Fluorescent LAMP&RT LAMP Kit (with UDG)

210811WarmStart-Fluorescent-LAMP&RT-LAMP-Kit-(with-UDG)_큰

기존 제품 E1700, WarmStart® LAMP Kit (DNA & RNA)LAMP 2X Master Mix와 LAMP Fluorescent Dye로 구성되어 있어 형광을 통해 LAMP/RT-LAMP 반응을 실시간으로 측정할 수 있습니다. 신제품 E1708, WarmStart® Fluorescent LAMP/RT-LAMP Kit (with UDG)는 E1700에 UDG가 포함되어 있어 carryover 오염을 방지할 수 있습니다. LAMP Fluorescent Dye 없이 LAMP/RT-LAMP 2X Master Mix (with UDG)만 필요한 경우 M1708을 선택해 주세요. 

WarmStart LAMP Kit (DNA & RNA) 

  • Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
  • Compatible with multiple detection methods
  • WarmStart® technology inhibits activity at room temperature

 WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG)

  • Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
  • Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
  • Includes fluorescent dye to enable real-time fluorescence measurement of LAMP/RT-LAMP reaction

WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG)

  • Simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets
  • Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the mix
  • Fully buffered master mix is compatible with different sample types and supports multiple detection methods
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Figure 1: The WarmStart LAMP Kit (DNA & RNA) is compatible with multiple detection methods

The WarmStart LAMP Kit (DNA & RNA) (NEB #E1700) includes fluorescent dye for real-time fluorescence measurement. Alternately, detection can be accomplished by turbidity detection or a variety of endpoint visualization methods. For carryover contamination prevention, a version containing dUTP and thermolabile UDG is available: WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) (NEB #E1708).

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Figure 2: The WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) provides robust detection of human DNA and RNA targets

RT-LAMP (RNA targets) or LAMP (DNA targets) experiments were performed with NEB #M1700: WarmStart LAMP 2X Master Mix, which is the master mix in the NEB #E1700 kit that does not contain dUTP/UDG, and NEB #M1708: WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG). Reactions containing 1X LAMP primers and 1X LAMP Fluorescent dye were set up in quadruplicate over three logs of total Jurkat RNA or Jurkat DNA (10 ng to 0.1 ng) in 96-well, 25 µl reactions. Control reactions without template (NTC) were also evaluated.  Reactions were incubated at 65°C for 40 minutes and fluorescence was monitored every 15 seconds in the SYBR/FAM channel of a real-time thermocycler (Bio-Rad® CFX96). Each dot represents the time at which the fluorescence signal for a single reaction crosses the instrument-defined threshold.  All four replicates were detected at each template input unless otherwise indicated (note that dots frequently overlap given similar detection time for the replicates). Overall, similar performance was observed for both NEB #M1700 and NEB #M1708 at each template input.No amplification was observed in any of the no template control reactions.

  • Related Products

The WarmStart Colorimetric LAMP 2X Master Mix is an optimized formulation of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx in a special low-buffer reaction solution containing a visible pH indicator for rapid and easy detection of Loop-Mediated Isothermal Amplification (LAMP) and RT-LAMP reactions.

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Negative reactions are indicated in pink and positive reactions are indicated by a change to yellow.

WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) 

  • Fast, clear pink-to-yellow visible detection of amplification
  • WarmStart® feature inhibits activity at room temperature
  • Learn how Colorimetric LAMP was used for rapid detection of coronavirus (COVID-19) RNA in our recent publication

WarmStart® Colorimetric LAMP 2X Master Mix with UDG 

  • Fast, clear pink-to-yellow visible detection of amplification
  • Reduce risk of carryover contamination with thermolabile UDG and dUTP included in the master mix
  • WarmStart® feature inhibits enzyme activity at room temperature allowing for flexible reaction setup
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