[NEB] NEBridge™ Ligase Master Mix

NEB_NEBridge_Ligase_Master_Mix

기존 NEB® Golden Gate Assembly Kit는 Type IIS restriction enzyme BsmBI-v2 (or BsaI-HF®v2)와 T4 DNA ligase가 mix 되어 있어 DNA 단편만 있으면 사용 가능한 제품입니다. 신제품 NEBridge™ Ligase Master Mix는 Type IIS 제한효소가 포함되지 않은 3X master mix (T4 DNA Ligase, optimized reaction buffer with ligation enhancer)로 Type IIS restriction enzymes을 선택해서 사용하실 수 있는 제품입니다. 

  • Optimized for efficient and accurate Golden Gate Assembly
  • Convenient 3X Master Mix format
  • Use with NEB Type IIS restriction enzymes
  • Ideal for ordered assembly of multiple fragments (2-25+) in a single reaction
  • Free tool available at GoldenGate.neb.com
  • Try our free Ligase Fidelity Tools ( for the design of high-fidelity Golden Gate Assemblies)
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Workflow for NEBridge Ligase Master Mix

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Suggested Type IIS Restriction Enzyme Amounts

  • Related Products

NEB® Golden Gate Assembly Kit (BsaI-HF®v2) 

  • Updated to include BsaI-HFv2 (optimized for Golden Gate)
  • Includes destination plasmid with T7/SP6 promoters
  • Compatible with a broad range of fragment sizes (< 100 bp to > 15 kb)

NEB® Golden Gate Assembly Kit (BsmBI-v2)

  • Uses BsmBI-v2, which is optimized for Golden Gate Assembly
  • Includes destination plasmid with T7/SP6 promoters
  • Compatible with a broad range of fragment sizes (< 100 bp to > 15 kb)
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