기존 NEB® Golden Gate Assembly Kit는 Type IIS restriction enzyme BsmBI-v2 (or BsaI-HF®v2)와 T4 DNA ligase가 mix 되어 있어 DNA 단편만 있으면 사용 가능한 제품입니다. 신제품 NEBridge™ Ligase Master Mix는 Type IIS 제한효소가 포함되지 않은 3X master mix (T4 DNA Ligase, optimized reaction buffer with ligation enhancer)로 Type IIS restriction enzymes을 선택해서 사용하실 수 있는 제품입니다.
- Optimized for efficient and accurate Golden Gate Assembly
- Convenient 3X Master Mix format
- Use with NEB Type IIS restriction enzymes
- Ideal for ordered assembly of multiple fragments (2-25+) in a single reaction
- Free tool available at GoldenGate.neb.com
- Try our free Ligase Fidelity Tools ( for the design of high-fidelity Golden Gate Assemblies)
Workflow for NEBridge Ligase Master Mix
Suggested Type IIS Restriction Enzyme Amounts
NEB® Golden Gate Assembly Kit (BsaI-HF®v2)
- Updated to include BsaI-HFv2 (optimized for Golden Gate)
- Includes destination plasmid with T7/SP6 promoters
- Compatible with a broad range of fragment sizes (< 100 bp to > 15 kb)
NEB® Golden Gate Assembly Kit (BsmBI-v2)
- Uses BsmBI-v2, which is optimized for Golden Gate Assembly
- Includes destination plasmid with T7/SP6 promoters
- Compatible with a broad range of fragment sizes (< 100 bp to > 15 kb)