[NEB] NEBNext® Immune Sequencing Kits (Human/Mouse)

NEBNext® Immune Sequencing Kit는 인간과 생쥐의 T cell 또는 B cell의 receptor repertoire 분석을 위한 제품으로 B-cell receptor/antibody (BCR)와 T-cell receptor (TCR) RNA transcripts의 enrichment & sequencing을 위한 시약으로 구성되어 있습니다. Unique molecular identifiers (UMIs) barcode 사용으로 PCR bias를 최소화하고 sequencing 정확도를 향상시켰습니다.

Figure 1: Simplified representation of the structure of an antibody or TCR

Figure 2: Workflow

NEBNext® Immune Sequencing Kit (Human), sequence the full-length immune gene repertoires of B cells and T cells. Profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes IgM, IgD, IgG, IgA, and IgE) with improved sequence accuracy. 

NEBNext® Immune Sequencing Kit (Mouse), sequence the full-length immune gene repertoires of B cells and T cells. Profile somatic mutations across all relevant contexts (e.g., V, D, and J segments and isotypes IgM, IgD, IgG, IgA, and IgE) with improved sequence accuracy.

Video Library: Using large scale B cell receptor repertoire sequencing to further understand human autoimmune disease

NEBNext Immune Sequencing data analysis user guide