NEB Luna® Probe One-Step RT-qPCR 4X Mix with UDG (M3019)*의 동결건조 제품이 새로 출시되었습니다.
* Luna® Probe One-Step RT-qPCR 4X Mix with UDG (M3019)
- cDNA 합성과 PCR 반응을 하나의 tube내에서 진행 가능한 4x Master Mix 타입의 One-Step RT-qPCR Kit (hydrolysis probe 방식)
- 4X master mix에 UDG가 포함되어 있어 carryover 오염 방지
신제품 LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG (L4001S)
- Simply add nuclease-free water for rapid rehydration (view protocol)
- Store at room temperature for up to 2 years prior to rehydration
- Prevent carryover contamination with Thermolabile UDG and dUTP included in optimized mix
- Use with a variety of instrument platforms, including those that require a high or low ROX reference signal
Figure 1: LyoPrime Luna offers a robust, versatile RT-qPCR option in a shelf-stable lyophilized format
The LyoPrime Luna™ RT-qPCR Mix (NEB #L4001) offers the same versatile features as the Luna RT-qPCR 4X Mix (NEB #M3019) in a lyophilized format that can be shipped and stored at room temperature.
Figure 2: Lyophilized and liquid Luna RT-qPCR mixes demonstrate equivalent strong performance
A. RT-qPCR targeting human β-actin was performed using either the LyoPrime™ Luna Probe One-Step RT-qPCR Mix with UDG (NEB #L4001) or Luna® Probe One-Step 4X Mix with UDG (NEB #M3019) over an 8-log range of input template concentrations (1 μg – 0.1 pg Jurkat total RNA) with 4 replicates at each concentration, run on an ABI® QuantStudio™ 6 Flex real-time instrument. Reactions (20 μl) included primers at 400 nM each and and probe at 200 nM, and followed the product recommended cycling conditions. B. Standard Curve results were substantially equivalent for the lyophilized (gold) and liquid-format (blue) mixes, with strong linearity and reproducibility observed, even at the lowest concentrations tested.
