Choosing the Right Reagents for your Coronavirus Immunoassay
References
- Severe Acute Respiratory Syndrome Coronavirus, NCBI.
- Coronavirus. Centers for Disease Control and Prevention (CDC), 2019.
- SARS (Severe Acute Respiratory Syndrome). World Health Organization
- Anthony et al. (2017). Further Evidence for Bats as the Evolutionary Source of Middle East Respiratory Syndrome Coronavirus. Bio. 4;8(2).
- Wang et al. (2015). Evaluation of candidate vaccine approaches for MERS-CoV. Nat Commun. 28;6:7712.
- Reusken et al. (2013). Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study. Lancet Infect Dis. 13(10):859-66.
- Al-Amri et al. (2017). Immunogenicity of Candidate MERS-CoV DNA Vaccines Based on the Spike Protein. Sci Rep. 23;7:44875.
- Middle East respiratory syndrome coronavirus (MERS-CoV). World Health Organization.
- Xu et al. (2019). Antibodies and vaccines against Middle East respiratory syndrome coronavirus. Emerg Microbes Infect. 8(1): 841–856.
- Novel coronavirus (2019-nCoV), World health Organisation (WHO), 2020.
- Hui et al. (2020). The continuing 2019-nCoV epidemic threat of novel coronaviruses to global health – The latest 2019 novel coronavirus outbreak in Wuhan, China. International Journal of Infectious Diseases, 91, 264–266.
- Lu R, Zhao X, Li J, et al. (2020). Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. S0140-6736(20)30251-8. doi:10.1016/S0140-6736(20)30251-8.
| Antigens | Product Code | Source | Format |
| SARS-CoV-2 Spike (Full-Length) | REC31868 | CHO Recombinant | Liquid |
| SARS-CoV-2 Spike S1 | REC31806 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 Spike S1 | REC31828 | Insect Cell Recombinant | Liquid |
| SARS-CoV-2 Spike S1 NTD | REC31835 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 Spike S2 | REC31807 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 Spike S2 | REC31830 | Insect Cell Recombinant | Liquid |
| SARS-CoV-2 Spike S2 (1000-1200) | REC31846 | E. coli Recombinant | Liquid |
| SARS-CoV-2 Spike S2 (800-1000) | REC31844 | E. coli Recombinant | Liquid |
| SARS-CoV-2 (B.1.1.529) Stabilized Spike | REC32008 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.1.529) Spike S1 | REC32006 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.1.529) Spike RBD | REC32007 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (501.V2) Stabilized Spike (HexaPro, Furin) | REC31926 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (501.V2) Spike RBD | REC31945 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.1.7) Spike (Full-Length) | REC31923 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.1.7) Stabilized Spike | REC31924 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.1.28) Stabilized Spike | REC31944 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.1.28: K417T, E484K, N501Y) Spike RBD | REC31961 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.351) Stabilized Spike (HexaPro, Furin) | REC31963 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.617.1: E484Q, L452R) Spike RBD | REC31971 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.617.1: L452R, T478K) Spike RBD | REC31974 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.617.2) Spike RBD | REC31974 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (B.1.617.2; AY.1, AY.2, AY.3) Spike | REC31975 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G) Spike (Trimer) | REC31898 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G, E484K) Spike S1 | REC31902 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G, G485R) Spike S1 | REC31901 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G, L84I, N439K) Spike S1 | REC31900 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G, N501Y) Spike S1 | REC31930 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G, S477N) Spike S1 | REC31899 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G, V445I, H655Y, E583D) Spike S1 | REC31903 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (N439K) Spike S1 | REC31933 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (N501Y) Spike S1 | REC31946 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (D614G) Spike S1 | REC31883 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 (A222V) Spike S1 | REC31932 | HEK293 Recombinant | Liquid |
| SARS-CoV-2 Nucleoprotein | REC31812 | E. coli Recombinant | Liquid |
| SARS-CoV-2 RBD-Nucleoprotein Chimera | REC31881 | CHO Recombinant | Liquid |
| SARS-CoV-2 Spike-Envelope-Membrane Mosaic | REC31829 | E. coli Recombinant | Liquid |
| SARS-CoV-2 3C-Like Proteinase (3CLpro), Active | REC31875 | E. coli Recombinant | Liquid |
| SARS-CoV-2 Papain-Like Protease (PLpro), Active | REC31873 | E. coli Recombinant | Liquid |
| SARS-CoV-2 RNA-Dependent Pol. (RdRp), Active | REC31874 | E. coli Recombinant | Liquid |
| SARS-CoV-2 Purified Viral Lysate | NAT41605 | VeroE6 Native | Liquid |
| Antibodies | Product Code | Source | Format |
| Mouse SARS-CoV-2 Spike RBD IgG mAb (FG11) | MAB12445 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike RBD IgG mAb (DH6) | MAB12444 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike RBD IgG mAb (CE5) | MAB12443 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S1 IgG mAb (HH10) | MAB12442 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S1 IgG mAb (FH4) | MAB12441 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S1 IgG mAb (EB5) | MAB12440 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (HA11) | MAB12448 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (AD10) | MAB12446 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (CH1) | MAB12447 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (JC7) | MAB12449 | Mouse Hybridoma | Liquid |
| Human SARS-CoV-2 Spike S1 IgG mAb (CR3022) | MAB12422 | Humanised Recombinant | Liquid |
| Human SARS-CoV-2 Spike S1 IgM mAb (CR3022) | MAB12423 | Humanised Recombinant | Liquid |
| Rabbit SARS-CoV-2 Spike S1 IgG mAb (CR3022) | MAB12424 | Rabbit Hybridoma | Liquid |
| Llama (VHH) SARS-CoV-2 Spike RBD IgG nAb (HL10) | MAB12472 | Llama Recombinant | Liquid |
| Llama (VHH) SARS-CoV-2 Spike RBD IgG nAb (HL14) | MAB12469 | Llama Recombinant | Liquid |
| Llama (VHH) SARS-CoV-2 Spike RBD IgG nAb (HL25) | MAB12470 | Llama Recombinant | Liquid |
| Llama (VHH) SARS-CoV-2 Spike RBD nAb (HL38) | MAB12471 | Llama Recombinant | Liquid |
| Llama (VHH) SARS-CoV-2 Spike RBD IgG nAb (L14) | MAB12473 | Llama Recombinant | Liquid |
| Llama (VHH) SARS-CoV-2 Spike RBD IgG nAb (L25) | MAB12474 | Llama Recombinant | Liquid |
| Llama (VHH) SARS-CoV-2 Spike RBD IgG nAb (L38) | MAB12475 | Llama Recombinant | Liquid |
| Human SARS-CoV-2 Spike IgA mAb (A60H) | MAB12439 | Humanised Recombinant | Liquid |
| Rabbit SARS-CoV-2 Spike S1 pAb | PAB21471 | Rabbit Sera | Liquid |
| Mouse SARS-CoV-2 Spike RBD IgG mAb (FG11) | MAB12445 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike RBD IgG mAb (DH6) | MAB12444 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike RBD IgG mAb (CE5) | MAB12443 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S1 IgG mAb (HH10) | MAB12442 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S1 IgG mAb (FH4) | MAB12441 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S1 IgG mAb (EB5) | MAB12440 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (HA11) | MAB12448 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (AD10) | MAB12446 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (CH1) | MAB12447 | Mouse Hybridoma | Liquid |
| Mouse SARS-CoV-2 Spike S2 IgG mAb (JC7) | MAB12449 | Mouse Hybridoma | Liquid |
| Receptors | Product Code | Source | Format |
| Human ACE2 (18-615) | REC31832 | HEK293 Recombinant | Liquid |
| Human ACE2 (19-740) | REC31833 | HEK293 Recombinant | Liquid |
| Human CD147 (22-205) | REC31872 | HEK293 Recombinant | Liquid |
| Human CLEC4M | REC31686 | HEK293 Recombinant | Liquid |
| Neutralisation Assay Development Kit | Product Code | Type | Format |
| SARS-CoV-2 Wild-Type | NTRL-RBD-10 | RBD-ACE2 NTRL Kit | Liquid |
| SARS-CoV-2 Alpha Variant (B.1.1.7) | NTRL-UK-RBD-10 | RBD-ACE2 NTRL Kit | Liquid |
| SARS-CoV-2 Beta Variant (501Y.V2) | NTRL-SA-RBD-10 | RBD-ACE2 NTRL Kit | Liquid |
| SARS-CoV-2 Delta Variant (B.1.617.2) | NTRL-DT-RBD-10 | RBD-ACE2 NTRL Kit | Liquid |
| SARS-CoV-2 Gamma Variant (B.1.1.28) | NTRL-BZ-RBD-10 | RBD-ACE2 NTRL Kit | Liquid |
| SARS-CoV-2 Kerala Variant (B.1.617.1) | NTRL-IN-RBD-10 | RBD-ACE2 NTRL Kit | Liquid |
| *SARS-CoV-2 Omicron Variant (B.1.1.529) | NTRL-OMI-SPIKE-10 | Spike-ACE2 NTRL Kit | Liquid |
| SARS-CoV-2 Dominant Lineage | Spike Mutations | Antigen | Product Code |
| Omicron Variant (B.1.1.529) | A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F | Pre-Fusion Stabilised Whole Spike | REC32008 >>> |
| Omicron Variant (B.1.1.529) | G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H | Spike Receptor-Binding Domain | REC32007 >>> |
| Omicron Variant (B.1.1.529) | A67V, Δ69-70, T95I, G142D, Δ143-145, Δ211, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y | Spike S1 Subunit | REC32006 >>> |
| Wuhan-Hu-1 Wild-Type | N/A | Pre-Fusion Stabilised Whole Spike | REC31966 >>> |
| Wuhan-Hu-1 Wild-Type | N/A | Spike Receptor-Binding Domain | REC31882 >>> |
| Alpha Variant (B.1.1.7) | 69-70del, Y144del, N501Y, A570D, P681H, T716I, S982A, D1118H | Pre-Fusion Stabilised Whole Spike | REC31924 >>> |
| Alpha Variant (B.1.1.7) | N501Y | Spike Receptor-Binding Domain | REC31946 >>> |
| Beta Variant (B.351.1/501Y.V2) | L18F, D80A, D215G, del242-244, K417N, E484K, N501Y, D614G, A701V | Pre-Fusion Stabilised Whole Spike | REC31963 >>> |
| Beta Variant (B.1.351/501Y.V2) | D80A, D215G, K417N, E484K, N501Y, A701V | Pre-Fusion Stabilised Whole Spike | REC31926 >>> |
| Beta Variant (B.1.351/501Y.V2) | K417N, E484K, N501Y | Spike Receptor-Binding Domain | REC31945 >>> |
| Delta Variant (B.1.617.2) | T19R, T95I, G142D, E156G, del157-158, W258L, N417K, L452R, T478K, D614G, P681R, D950N | Pre-Fusion Stabilised Whole Spike | REC31975 >>> |
| Delta Variant (B.1.617.2) | L452R, T478K | Spike Receptor-Binding Domain | REC31974 >>> |
| Gamma Variant (B.1.1.28) | L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F | Pre-Fusion Stabilised Whole Spike | REC31944 >>> |
| Gamma Variant (B.1.1.28) | K417T, E484K, N501Y | Spike Receptor-Binding Domain | REC31961 >>> |
| Kappa Variant (B.1.617.1) | E484Q, L452R | Spike Receptor-Binding Domain | REC31971 >>> |
| Lambda Variant (C.37) | G75V, T76I, 246-252del, L452Q, F490S, D614G, T859N | Pre-Fusion Stabilised Whole Spike | REC31976 >>> |
| Lambda Variant (C.37) | L452Q, F490S | Spike Receptor-Binding Domain | REC31992 >>> |
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