LNPs Beyond mRNA Vaccines: Where Do We Go from Here?
Lipid Nanoparticles(LNPs)은 현대 의학에 혁신을 일으킬 준비가 되어 있습니다. 이 기술은 전염병을 넘어 한 때는 감당할 수 없을 것으로 여겨졌던 target에 도달할 수 있을 만큼 진화했고, 거의 무한한 수의 질환을 치료할 수 있는 엄청난 가능성을 지니고 있습니다. 이 분야는 아직 초기 단계에 있으며, Cayman Currents의 이번 호에서는 현대 의학의 선두로 나아가는 LNPs의 지속적인 발견과 혁신을 소개하고 있으며, Cayman에서 제공하는 LNP 연구를 지원하기 위한 제품과 tool들을 소개하고 있습니다.
페이지를 아래로 내리면 Cayman에서 발행한 Lipid Nanoparticles 단행본 (Issue 36 l FALL 2023)을 다운로드 할 수 있습니다.
Self LNP formulation을 원하시는 고객들을 위한 Lipid Products
Fixed and ionizable cationic lipids are used in LNPs to encapsulate nucleic acid and promote endosomal escape. (Read the article)
SM-102 (Item No. 33474)
SM-102 was brought to the forefront of LNPs during the COVID-19 pandemic. It is one of the components of the mRNA-1273 COVID-19 vaccine. Cayman was the first company to offer research-grade SM-102 during the COVID-19 pandemic.
ALC-0315 (Item No. 34337)
ALC-0315 was made famous through its use in mRNA-based COVID-19 vaccines. It is one of the components of the BNT162b2 COVID-19 vaccine.
DLin-MC3-DMA (Item No. 34364)
DLin-MC3-DMA was used in the first FDA-approved nucleic acid therapy packaged in an LNP.
RCB-4-8 (Item No. 38803)
RCB-4-8-containing LNPs improve lung transfection efficiency by approximately 100-fold over MC3-containing LNPs in mice. RCB-4-8 may be useful for CRISPR applications.
Anionic lipids prevent particle aggregation during storage and can be used to modify the net surface charge of the LNP.
Neutral phospholipids have a role in membrane fusion and can be used to modify the net surface charge of the LNP.
1-Palmitoyl-2-Oleoyl-sn-glycero-3-PC
(Item No. 15102)
1,2-POPC is a neutral phospholipid containing palmitic acid and oleic acid at the sn-1 and sn-2 positions, respectively, and a phosphatidylcholine (PC) head group.
1,2-Distearoyl-sn-glycero-3-PC
(Item No. 15100)
1,2-DSPC is a neutral phospholipid containing stearic acid at the sn-1 and sn-2 positions and a PC head group. It is a component of both US FDA-approved mRNA COVID-19 vaccines.
PEGylated lipids prevent opsonization, improving particle stability and increasing bioavailability.
ALC-0159 (Item No. 34336)
ALC-0159 is formed by PEGylation of N,N-dimyristylamide of 2-hydroxyacetic acid. It is one of the components of the BNT162b2 COVID-19 vaccine.
DMG-PEG(2000) (Item No. 33945)
DMG-PEG(2000) is formed by PEGylation of dimyristoyl glycerol. It is one of the components of the mRNA-1273 COVID-19 vaccine.
Sterol lipids provide stability to LNPs and promote membrane fusion and endosomal escape.
Cholesterol (Item No. 9003100)
Cholesterol interacts with membrane phospholipids, sphingolipids, and proteins to influence their behavior. In LNPs, it has a role in stability and cellular uptake. It is a component of both US FDA-approved mRNA COVID-19 vaccines.
DC-Chol (hydrochloride) (Item No. 16943)
DC-Chol is a cationic cholesterol derivative. As a component of lipoplexes with 1,2-DOPE, it has been used for transfection of mRNA without affecting cell viability.
Custom service를 원하시는 고객들을 위한 "Cayman's LNP Development Services"
Our interdisciplinary team of scientists has expertise in lipid chemistry and custom synthesis, cell and molecular biology, assay development, immunology, and more to help customize an LNP development program to meet your project goals.
Cayman의 scientist들은 여러분의 프로젝트와 목표에 대해 함께 논의를 통해, 여러분의 cargo, 조직 또는 세포 유형에 적합한 최적의 lipid LNP 구성 요소와 실험 설계를 결정할 수 있도록 도와드립니다.
| Cargo | Lipids |
| Clients supply their own nucleic acid cargo, choose from one of several reporter options we offer, or provide a sequence for outsourced custom synthesis. | Select from more than 200 ready-made lipids for LNPs or rely on our chemical synthesis experts for design and synthesis of custom lipids. |
Microfluidic formulation using variable flow rates and lipid ratios for screening LNPs or bulk preparation of R&D-optimized LNPs.
Analysis of LNP and payload concentration, particle size, polydispersity index, pKa, stability, aggregation, and encapsulation efficiency.
Bioanalytical services for in vitro assessment of payload delivery and downstream biological effects.
- Ready-made assays and custom assay development with high-throughput options available.
- Flow cytometry and high-content imaging readout of reporter payload delivery and/or expression, effects on cell viability.
- Cell-based, immunoassays, enzyme activity, reporter, mass spectrometry, ELISpot, and immunophenotyping assays to measure the impacts of therapeutic payload delivery.
- BSL2+ cell culture for immortalized or primary cells.
Cayman GMP 담당 부서와 함께 여러분의 LNPs를 다음 단계로 이끌어 보세요. 미국에 본사를 둔 GMP 팀은 여러분의 formulation을 위한 lipid 성분의 제조 및 scale-up을 최적화할 수 있으며, 최상의 lipid를 보장하고 인체 시험용 LNP 생산을 위해 여러분의 CDMO 파트너로써 원활한 이행을 지원합니다.
Cayman Helps Make Research Possible
Cayman Chemical has been supplying high-purity lipids to the scientific community for over 40 years, and our industry-leading expertise in lipid chemistry, synthesis, and purification is upported by state-of-the-art analytical equipment. We are committed to offering an unprecedented portfolio of LNP research tools. We offer an impressive collection of Cayman Helps Make Research Possible of multiple approaches to address the current limitations of LNPs and collaborative R&D efforts to draw from the extensive expertise across scientific disciplines to bring LNPs to the forefront of modern medicine. ready-to-use lipids for LNPs and research-ready LNPs to screen different LNP compositions along with in-house lipid synthesis, screening, and LNP development services.
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