IMMUNO-ONCOLOGY RESEARCH TOOLS
최근 Cancer immuno-therapy가 암치료 분야에서 가장 주목 받고 있는 신기술로 대두되면서 면역 항암 연구(immuno-oncology) 또한 빠르게 성장하고 있습니다. 면역조절제 (immunomodularoty drugs)와 생물제제 (biologics)의 개발 및 연구를 위해서는 인위적으로 면역 활성을 유도할 수 있는 다양한 human cell-based model이 필요합니다. 이에 ATCC는 다양한 방법으로 검증된 cell lines, human primary immune cells과 advanced cell models을 제공하고 있습니다.
Primary Human Immune Cells
♦ Cancer immunology studies ♦ Toxicity screening ♦ Transplantation and graft rejection ♦ Inflammation and allergy ♦ Vaccine and drug development
ATCC scientists have conducted in-depth authentication and quality control analyses on each of the primary immune cell types. In addition, the utility of these cells for scientific studies has been confirmed by ATCC R&D scientists. For example, the differentiation capacity of the bone marrow CD34+ cells and the peripheral blood CD14+ monocytes was characterized. Additionally, to confirm their immune activity the peripheral blood CD56+ natural killer cells were utilized as the effector cells in an NK activity assay.
Continuous Cell Lines
ATCC houses a vast collection of cell lines derived from various normal and diseased tissues from multiple species, representing a variety of immunological cells. ATCC routinely authenticates its cell lines using the following methods:
♦ Short tandem repeat (STR) profiling, to establish a DNA fingerprint ♦ Cellular morphology, which is monitored for consistency
♦ Cytochrome C Oxidase I (COI) Assay, for species determination ♦ PCR testing, for mycoplasma detection
Tumor & Immune cell에 발현된 checkpoint inhibitory & co-stimulatory molecules에 대한 Full-version 자료가 필요하시면 아래 첨부 파일을 클릭해 주세요
THP-1 Reporter Cells
THP-1 luciferase reporter cell Lines은 NFκB, cAMP/PKA, MAPK/ERK 등 immune signaling pathway의 key transcription factor의 response element promoter에 의해 Luciferase 유전자가 발현되도록 THP-1 세포에 LUC2 유전자를 lentiviral transduction 후 single cell clonal selection 한 제품입니다.
| Key Features | Key Benefits | |
| Fully authenticated parental THP-1 cell line | No concerns about cross-contamination and misidentification, saves time and money | |
| High signal-to-noise ratio | Clear and more intense results, straightforward data analysis | |
| Verified, characterized stable expression | Reduced variability, reproducible results | |
| Easy to culture, robust, and highly sensitive | Ease of use, customer convenience | |
| Amenable to complex experimentation (combinatorial stimulation, co-culture) | Versatile and compatible with multiple platforms | |
| High density cryopreservation | More viable cells post-thaw | |
Table 1. Available THP-1 LUC2 reporter cell lines (cat#를 클릭하시면 상제 제품 페이지로 이동합니다)
| Response Element | ATCC No. | Signaling Pathway | Function | ||||||||
| NFκB | TIB-202-NFκB-LUC2™ | NFκB | Pivotal mediator of inflammatory response | ||||||||
| GAS | TIB-202-GAS-LUC2™ | JAK-STAT (Type II) | Initiates immune cell activation and recruitment | ||||||||
| CRE | TIB-202-CRE-LUC2™ | cAMP/PKA | Inflammatory mediator and phagocytosis modulator | ||||||||
| ISRE | TIB-202-ISRE-LUC2™ | JAK-STAT (Type I) | Initiates immune cell activation and recruitment | ||||||||
| AP1 | TIB-202-AP1-LUC2™ | MAPK/ERK | Regulates innate and adaptive immune response | ||||||||
| NFAT | TIB-202-NFAT-LUC2™ | Calcineurin-NFAT | Mediates adaptive T and B cell activation | ||||||||
Fig.1: Quantitation of immunomodulation made easy. To use THP-1 LUC2 cells, simply seed in a 96-well plate. Stimulate the cells overnight with your compound of interest, then incubate the cells using a luciferase assay system and read the bioluminescence signals using a luminometer. Your immunomodulation data will be bigger, brighter, better.
THP-1 Reporter Cells에 대해 더 자세한 내용이 궁금하시면 아래 첨부 파일을 클릭하세요
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