ANASPEC에서는 25년 이상의 전문적인 peptides 합성 경험, 고객 협업 및 엄격한 품질 기준으로 최적화된 peptides를 제공하고 있으며, 약물개발 및 단백질체 연구를 포함한 다양한 응용분야에 충족하는 맞춤형 펩타이드 합성을 위한 versatile platform을 제공합니다. 고품질의 다양한 맞춤형 제품들을 경험해 보세요.
Custom Peptides :
Custom peptide Request
Heavy Isotope-labeled Peptides
Peptide Libraries
Fluorescent Dye Labeled Peptides
FRET Peptides
TR-FRET Peptides
Stapled Peptides
Cyclic Peptides
Disulfide-Bridged Peptides
Glycosylated and Glycated Peptides
Lipopeptides
Phosphorylated Peptides
Peptide-Oligo Conjugates
Peptide Modifications
High Quality Peptide Synthesis :
GENERAL SPECIFICATIONS
Length
Up to 60 amino acids
Purity
≥95%, ≥90%, ≥85%, ≥70%, or crude
Quantity
1mg minimum up to gram quantities, delivered as gross weight or net weight
Counter-Ion
Default is TFA salt. We also offer acetate, chloride or ammonium salt exchange for an additional fee
Please note that these specifications may vary for modified peptides
QC TESTING
Identity
By Mass Spec Analysis
% Purity
By HPLC
Additional testing available upon request:
- Water content determination
- Peptide content determination (CHN analysis or quant-peptide service)
- Solubility testing
- Peptide Sequence confirmation
- Counterion determination
- Heavy metal content determination
- Endotoxin & Bioburden content determination
DOCUMENTATION & DELIVERY
Documentation
- HPLC & Mass Spec chromatograms
- Technical data sheet (TDS)
Format
Lyophilized powder, peptide in solution on request
For special format, please inquire about our Dispensing options
Shipping
Ambient temperature
Lead Time
4-6 weeks
Highly modified peptides may require longer production times
Purity vs Applications
Applications |
• Blocking and competition assays • Enzyme-substrate studies (quantitative) • Receptor-ligand interaction studies (quantitative) • ELISA and RIA (quantitative) • in vivo/in vitro studies • High precision quantitative proteomics |
• Western blotting studies • Enzyme-substrate studies • Phosphorylation studies • Affinity purification |
• Production of antibodies • Determination of the titer |
Recommended purity level |
≥ 90-95% | ≥ 80 – 85% | ≥ 70% |
Q&A :
How should I store my peptides?
Lyophilized peptides
For the long-term storage of peptides, we recommend that peptides be stored as a solid powder. Lyophilized peptides can be stored at temperatures of -20 °C or lower with little degradation. AnaSpec’s catalog peptides are checked every 2 years from the date of QC release to ensure that these peptides are within the required purity specification. AnaSpec recommends that customers also re-check their custom peptides every 2 years from the date of QC release.
Peptides in solution
Peptides in solution are much less stable and are susceptible to degradation. Solutions of water used to dissolve peptides should be sterile and purified.
In solution, some amount of the peptide may degrade depending on the amino acids present in the sequence. A few examples of which are shown below:
– Peptides containing methionine, cysteine, or tryptophan residues should have limited storage time in solution due to oxidation. These peptides should be dissolved in oxygen-free solvents.
– Glutamine and asparagine can deamidate to Glu and Asp, respectively.
– Cysteines can undergo oxidative cyclization to form Cys-Cys disulfide bridges (intra or inter disulfide bridges can form).
– Charged residues (Asp, Glu, Lys, Arg, His) are hygroscopic (take up water from moisture in the air) and will easily form a viscous clear oil. This physical change may not affect the properties of the peptide. It is recommended that such peptides be first lyophilized to remove trace amounts of water, degassed with N2 to ensure that there is no water vapor in the vial, and quickly capped.
To prevent any damage caused by repeated freeze-thaw cycles, we recommend that the user only dissolves the amount of peptide needed for the immediate experiment. Any excess solutions should be stored at ≥ -20 oC until needed.
Avoid moisture
As moisture will greatly reduce the long-term stability of peptides, we recommend that the peptide should be allowed to equilibrate to room temperature in a desiccator before opening the vial. Once the peptide has been dispensed, any remaining peptide in the tube should be gently purged with anhydrous nitrogen, the container recapped, sealed with parafilm, and stored at -20 °C.
How do I solubilize my peptides?
Peptide solubility characteristics vary strongly from one peptide to another. Residues such as Ala, Cys, Ile, Leu, Met, Phe, and Val will increase the chance of the peptide being hydrophobic and dissolving in aqueous solutions. AnaSpec catalog peptides are tested for their solubility in certain solvents. This information is located on every QC Datasheet that is shipped along with the peptide. AnaSpec recommends that customers adhere to the below guideline for their custom peptides.