[NEB] HiScribe™ T7 mRNA kit with CleanCap® Reagent AG

HiScribe™-T7-mRNA-Kit-with-CleanCap®-Reagent-AG

신제품 HiScribe™ T7 mRNA kit with CleanCap® Reagent AG는 RNA yield는 손상시키지 않으면서 single simplified reaction에서 natural Cap-1 structure를 포함하는 mRNA를 co-transcriptionally capping 하기 위해 최적화된 RNA synthesis formulation과 trinucleotide cap analog technology을 적용하였습니다. 이 키트에서 합성된 Cap-1 mRNA는 transfection, microinjection, in vitro translation, preclinical mRNA therapeutic mRNA studies 및 RNA 구조 및 기능 분석 등 다양한 응용 분야에서 사용할 수 있습니다. 

  • Generate up to 90 µg of Cap-1 mRNA per reaction from 1 µg of control template
  • NTPs are provided separately to enable partial of full substitution of modified NTPs
  • Template removal and RNA purification reagents included
  • Includes linearized control template for use with CleanCap® Reagent AG for verification of RNA synthesis

Figure 1. CleanCap Reagent AG

Figure_1._CleanCap_Reagent_AG

Figure 2. Schematic of CleanCap Reagent AG Promoter and Initiation Sequence

Figure_2._Schematic_of_CleanCap_Reagent_AG_Promoter_and_Initiation_Sequence

Figure 3. CleanCap Reagent AG results in higher mRNA synthesis yield than the  ARCA analog

Figure_3._CleanCap_Reagent_AG_results_in_higher_mRNA_synthesis_yield_than_the_ ARCA_analog

All reactions were performed with 5 mM CTP, 5 mM UTP and 6 mM ATP.  Standard IVT reactions included 5 mM GTP and no cap analog.  ARCA reactions contained a 4:1 ratio of ARCA:GTP (4mM:1mM).  IVT with CleanCap Reagent AG contained 5 mM GTP and 4 mM CleanCap Reagent AG and was performed according to recommended protocol (Standard mRNA Synthesis, HiScribe T7 mRNA Kit with CleanCap Reagent AG).  Reactions were incubated for 2 hours at 37°C, purified and quantified by NanoDrop®.

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