안녕하세요. 코람바이오텍(주) 학술팀입니다.
NEB Restriction enzymes 및 일부 DNA modifying enzymes의 BSA가 포함된 NEBuffer는 2021년 4월부터 점차적으로 아래와 같이 변경되었습니다. NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)와 rCutsmart™ Buffer는 BSA 대신 Recombinant Albumin (rAlbumin)을 대체한 New NEBuffer입니다.
기존 NEBuffer 1.1, 2.1, 3.1, CutSmart™ Buffer에는 동물 유래 Bovine Serum Albumin (BSA)가 첨가되어 있습니다.
|B7030S||NEBuffer™ Set (r1.1, r2.1, r3.1 and rCutSmart™)||10 X||1.25 ml each|
|B6002S||NEBuffer™ r2.1||10 X||4 x 1.25 ml|
|B6003S||NEBuffer™ r3.1||10 X||4 x 1.25 ml|
|B6004S||rCutSmart™ Buffer||10 X||4 x 1.25 ml|
Recombinant Albumin (rAlbumin) (NEB #B9200) is a non-bovine derived albumin that can serve as an alternative to Bovine Serum Albumin (BSA). Like BSA, it has been shown to prevent adhesion of enzymes to reaction tubes and pipette surfaces. It also stabilizes some proteins during incubation. Choose Recombinant Albumin when there is a need to avoid BSA.
In addition to being animal-free, a recombinant source of albumin offers increased consistency and purity. It eliminates sources of contamination and decreases lot-to-lot variability.
NEB has rigorously tested the performance between BSA- and rAlbumin-containing buffers and enzyme formulations (NEB #B9200). We have not seen a difference in enzyme performance when using either. Rest assured, you can continue to use either version for your experiments.
ClaI (NEB #R0197) restriction endonuclease was formulated with BSA or Recombinant Albumin (rAlbumin). Each of these formulations was tested using either A. CutSmart (contains BSA) or B. rCutSmart (contains rAlbumin) Buffer. Serial dilutions of ClaI were tested using Lambda DNA and the recommended assay conditions, followed by analysis using agarose gel electrophoresis. M is marker 1 kb Plus DNA Ladder (NEB #N3200). ClaI activity is the same in all formulation conditions tested. These representative data demonstrate equal performance between BSA and rAlbumin when used in both enzyme formulation and reaction buffer.
현재 보유중인 old tube NEBuffer 사용가능여부
BSA가 포함된 NEBuffer 또는 Recombinant Albumin(rAlbumin)(NEB #B9200)을 포함하는 NEBuffer를 사용할 때 효소 성능에 차이가 없기 때문에 BSA가 포함된 buffer를 사용할 수 있습니다. 하지만, 2022년부터 BSA가 포함된 buffers는 더 이상 판매하지 않습니다.
※ 자세한 내용은 NEB new buffer system 공지 사이트를 참고하시면 됩니다. (아래 링크 참조)
NEB new buffer system 공지 사이트