자주 묻는 질문 (FAQ)
Q1. cDNA clones의 gene expression이 보장되나요?
A1. ORIGENE사에서 판매하는 모든 cDNA clones은 홈페이지에 안내되어 있는 sequence가 일치함을 보장하며 gene expression은 보증되지 않습니다.
No. Every gene expression is different. Although the cDNA is driven by a strong promoter, mRNA can be expressed. However, a few factors can affect the steady state level of the protein of interest, including mRNA stability, protein translation efficiency and protein stability. Therefore, gene expression can not be guaranteed. This is the nature of genes. The sequence of a cDNA clone can be guaranteed to match the sequence posted on the website.
OriGene clone의 클레임은 잘못된 Sequence에 대해서만 가능합니다.
• Clone(TrueClone, TrueORF) : gene expression can not be guaranteed. This is the nature of genes. The sequence of a cDNA clone can be guaranteed to match the sequence posted on the website.
• TrueORF Gold : Please keep in mind that we guarantee the sequence of the clone posted on the website, and we guarantee the Western data of the clone expressed in HEK293 cells, and detected using a DDK antibody.
Q2. cDNA clones은 reference sequence와 일치 하나요?
A2. Sequence는 각 product page에서 확인할 수 있으며, NCBI reference와 다를 수 있기 때문에 구매하기 전에 확인이 필요합니다.


Q3. Tagging 된 GFP를 확인할 때 일반적인 GFP antibody를 사용해도 되나요?
A3. TrueORF clones에 사용된 GFP는 turboGFP로 일반적인 mGFP와는 다릅니다. ORIGENE의 anti-turboGFP antibody(TA150041)를 사용해야 합니다.
* turboGFP cannot be detected with antibodies to EGFP (assuming that is "normal" GFP). TurboGFP is a dimeric fluorescence protein we have licensed from Evrogen. mGFP는 monomeric GFP 입니다.
※ 참고
• TrueORF, TrueORF Gold는 GFP tagged (tGFP, TurboGFP)
• Lenti-ORF clones(Lentiviral vectors)는 mGFP tagged (mGFP, monomeric GFP)
• shRNA Vectors는 GFP tagged (tGFP, TurboGFP)