[2021 ATCC 웨비나] hTERT-immortalized Melanocytes – Advanced Models for Your Dermal Toxicity Studies

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hTERT (human TelomErase Reverse Transcriptase)-immortalized Cells이란 ?

hTERT 유전자를 human primary cell에 도입시켜 chromosome의 telomere 말단이 짧아지지 않도록 제작한 세포로, primary cell의 특성과 continuous cell line의 장점이 결합된 제품입니다.

Table 1. Comparison Between Primary, hTERT-immortalized, Oncogene/Viral Immortalized and Continuous Cell Lines.

  Primary Cells hTERT-immortalized Onco, Viral- Immortalized Continuous
Mimic in vivo Tissue Phenotype ++++ +++ ++ +
Karyotypic Stability Diploid Diploid/ Pseudodiploid Pseudodiploid/ Aneuploid Aneuploid
Proliferative Capacity + +++ +++ +++
Supply + +++ +++ +++
Inter-Experimental Reproducibility Low Good Good Good
Cost High Medium Low Low
Ease of Use + ++ ++ +++

 

ABSTRACT

hTERT-immortalized Melanocytes – Advanced Models for Your Dermal Toxicity Studies

Drug development and toxicology studies require physiologically relevant cell models that maintain key phenotypic features. Primary cells offer one such option; however, these physiological in vitro models are not without weaknesses including donor-donor variability, loss of certain in vivo functionalities, and limited lifespan. Here we present two immortalized melanocyte cell models – hTERT neonatal melanocytes and hTERT adult melanocytes – created by the transduction of human telomerase into primary melanocytes. These models have been tested and validated for longevity and to maintain primary cell features, such as melaninogenesis. Immortalized Melanocytes can be used for performing studies of the complex process of skin pigmentation, high throughput screening, environmental/cosmetic toxicology, and basic research into cell metabolism.

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