[Cell Signaling Technology] Protocol Compatibility Table for Immune Signaling and Phenotyping by Flow Cytometry

CST 혈류세포측정법에 의한 면역신호 및 표현형식의 프로토콜 호환성표

Cell Signaling Technology는 다양한 형광 dye와 결합된 flow-cytometry validated antibody 의 다양한 카탈로그를 제공합니다. 특히 flow-cytometry을 위해 multiplex panels에서 함께 사용할 여러 개의 antibody를 선택할 때 CST 카탈로그의 어떤 staining protocol과 antibody가 호환되는지 빠르게 식별할 수 있도록 immune signaling 및 phenotyping에 대한 protocol compatibility table을 제작하였습니다.

CST 혈류세포측정법에 의한 면역신호 및 표현형식의 프로토콜 호환성표

Protocol Overview

Protocol Information Brief Description of Staining Preparation Protocol Available Fixation-Permeabilization Kits (cat.#)
Live Cell Surface Staining Protocol Protocol where there is no fixation or permeabilization of the cells before staining Not Applicable
Fixed Cell Intracellular Staining – Methanol Permeabilization Protocol Uses formaldehyde and methanol for fixation and permeabilization of the cells before staining #13593
Fixed Cell Intracellular Staining – Triton™ X-100 Permeabilization Protocol Uses formaldehyde and Triton™ X-100 for fixation and permeabilization of the cells prior to staining #51995
Fixed Cell Intracellular Staining – FoxP3/Transcription Factor Protocol Fixation and permeabilization buffer set optimized for staining with antibodies to transcription factors and nuclear proteins #43481