Establishment of 3-D Neurosphere Culture from Human iPSC-Derived Neural Progenitor Cells
Human induced pluripotent stem cell (iPSC)–derived neurospheres 은 인간의 뇌 연구에 적합한 대표적 in vitro 모델입니다. 특히 희귀 유전병, 알츠하이머, 각종 면역 질환, host-pathogen interaction 및 환경 오염에 의한 독성 연구 등에 적용할 수 있습니다.
이 Application note는 ATCC derived neural progenitor cells (NPCs) 로부터 3D neurospheres in vitro 모델을 제작하는 방법과 dopaminergic differentiation과 drug toxicity assays 같은 experimental application 에 대해 설명하고 있습니다.
Table 1: Cells, media, and supplements used in neurosphere culture
| ATCC® No. | Designation | Notes |
| ACS-5001™ | Neural Progenitor Cells derived from Parkinson’s Disease Donor | NPCs derived from the human iPSC line ATCC-DYS0530 (ATCC® ACS-1013™) |
| ACS-5003™ | Neural Progenitor Cells derived from a normal donor | NPCs derived from the human iPSC line ATCC-BXS0117 (ATCC® ACS-1031™) |
| ACS-3003™ | NPC Growth Kit | A media kit designed for the growth and expansion of neural progenitor cells; add the contents of the kit to DMEM: F-12 Medium |
| ACS-3004™ | NPC Dopaminergic Differentiation Kit | A media kit designed for the dopaminergic differentiation of neural progenitor cells; add the contents of the kit to DMEM: F-12 Medium |
| ACS-3035™ | Cell Basement Membrane | The gel substrate required for establishing and expanding 2-D neural progenitor cell cultures |
| 30-2006™ | DMEM: F-12 Medium | The base medium for growth or differentiation media formulations |
| ACS-3020™ | Stem Cell Freezing Medium | A serum-free, xeno-free, complete medium qualified for the cryopreservation of neural progenitor cells |
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