Best Practices in Cryopreservation
Cryopreservation is the use of very low temperatures to structurally preserve intact living cells and tissues. Normally, the freezing of water in cells causes catastrophic damage to cellular structure by physical damage of ice formation and increased imbalance of solutes. Cryopreserving cells with the proper cryoprotectants and techniques will maximize viability of cells for cell culture. This webinar presentation will discuss best practices for cryopreservation focusing on determining optimal freezing rates and cryoprotectants, selecting proper containment units, managing a biorepository, and handling cells post-thaw. Emphasis will be placed on reviewing time-proven techniques while introducing newer innovative approaches to maximize reliability in cryopreservation for modern day cell culture.
